Metabolic pathway in vivoBiochemical metabolism in vivo Intracellular biochemical metabolismNutriBuilder® (Guanidinoacetic acid, GAA) is a high-tech green and efficient feed supplement, manufactured and developed by Beijing Gendone Biotechnology Co., Ltd. It concentrates more than 15-years research and knowledge from Gendone and Chinese agricultural scientists and has been widely accepted and used by local & foreign customers for more than 10 years.As the exclusive precursor of creatine, guanidinoacetic acid has been authorized by China, Europe and USA as a new feed additive, and widely proved with significant improvements in growth performance, carcass traits, meat quality and reproduction performance for swine.Guanidinoacetic acid (GAA) is the direct endogenous precursor of creatine in the organism of all vertebrates. Creatine biosynthesis is a two-step process. In the kidney, arginine transfers its amidino group to glycine to synthesize GAA. GAA is transferred to the liver, where S-adenosylmethionine (SAM) gives its methyl group to GAA to synthesize creatine. Creatine is then transported by the bloodstream to the target cells with high energy need, such as skeletal muscle, mammary gland, heart, brain, sperm and immune cells. Creatine plays a key role in energy transfer and supply via creatine-phosphocreatine system. Dietary supplemental NutriBuilder® can increase creatine level in the body by 20%. 1234CrCRTATPATPATPATPATPaseANTADPPCrADPADPOPADPANTmtCKGCK-gCKcCKa

Comparison of hygienic indexes among different brandsComparison of pork quality after using different brands of GAAItemGAA(%)Cyanamide(mg/kg)Dicyandiamide(mg/kg)Melamine(mg/kg)Dioxin(pg TEQ/kg)≥96%≤100≤2000≤5≤100≥96%≤300≤5000≤20≤750＜96%（variable）2000-200002000-1000020-2001000-5000NutriBuilder®Overseas brandLocal brand① Cyanamide, 300 mg/kg in GAA will cause weight loss, anemia, hypothyroidism, stooping back, tremors, rough fur, pale soft exudative(PSE) pork, nervous disorder and higher death rate in pigs.② Dicyandiamide, 2000 mg/kg in GAA degrades into gaseous cyanide access to stomach acid, causes and aggravates respiratory diseases for pigs. Besides, dicyandiamide tastes bitter and affects feed intake.③ Melamine, above 10 mg/kg in GAA reduces feed consumption, weight loss, bladder stones, crystalluria, renal failure and reduces survival rate of pigs.④ Dioxins, above 750 pg TEQ/kg in GAA are extremely harmful to human and animals. Human will suffer reproductive function damage and development problems, immune system damage and cancer after eating even low level of dioxins.2High content of GAA, strict quality control for residues. With a near density (550 kg/m3±10%) as corn and soybean, it can be easily premixed well with feedstuff.3VSInferior GAANutriBuilder®

Meat quality in finishing pigs (75-115 kg, 45 days)3.606.205.765.725.685.645.605.566.156.106.056.005.955.903.503.403.303.203.103.002.902.805.725.626.145.992.983.56Drip loss(%)pH45 minpH24 hControl0.05% NutriBuilder®Control0.05% NutriBuilder®Control0.05% NutriBuilder®Litter performance at birth (sows from gestation 103rd day to Lactation 21st day)Piglet performance during lactation (sows from gestation 103rd day to Lactation 21st day)For sowsDietary supplemental proper dose of NutriBuilder® in sows can increase born alive by 0.5-1.0 per litter, increase birth weight 40-50g and weaning average body weight by 200 g per piglet. Besides, NutriBuilder® can shorten sow labor, reduce back fat loss, improve survival rate of piglets and reduce the rate of weak piglets.10.5010.4010.3010.2010.1010.009.909.809.709.609.50195.00190.00185.00180.00175.00170.001.611.601.591.581.571.561.551.545.655.605.555.505.455.405.355.3096.0095.0094.0093.0092.0091.0090.0089.004.504.003.503.002.502.009.82180.48190.955.355.6191.1994.9210.36Born aliveBirth weight(kg)The rate of weak piglets(%)ADG(g)Weaning weight(kg)Survival rate(%)1.561.604.212.62Control0.06% NutriBuilder®Control0.06% NutriBuilder®Control0.06% NutriBuilder®Control0.06% NutriBuilder®Control0.06% NutriBuilder®Control0.06% NutriBuilder®

Beijing Gendone Biotechnology Co., Ltd.Address: Room 601, Block B, Gem Techcenter, Shangdi 3rd St., Haidian Dist., Beijing, ChinaZIP Code: 100085 TEL: +86-10-82890948 Email: overseadept@gendone.comGu an Gendone Biological Engineering Co., Ltd. Address: Southern Dist., Industrial Park, Gu an County, Langfang City, Hebei Province, ChinaZIP Code: 065500 TEL: +86-10-82890948http://www.gendone.comContact us