Metabolic pathway in vivoBiochemical metabolism in vivo Intracellular biochemical metabolismNutriBuilder® (Guanidinoacetic acid, GAA) is a high-tech green and efficient feed supplement, manufactured and developed by Beijing Gendone Biotechnology Co., Ltd. It concentrates more than 15-years research and knowledge from Gendone and Chinese agricultural scientists and has been widely accepted and used by local & foreign customers for more than 10 years.As the exclusive precursor of creatine, guanidinoacetic acid was authorized by China, Europe and USA as a new feed additive, and it has been widely proved with significant improvements in growth performance, carcass traits, laying and hatching performance for poultry.Guanidinoacetic acid (GAA) is the direct endogenous precursor of creatine in the organism of all vertebrates. Creatine biosynthesis is a two-step process. In the kidney, arginine transfers its amidino group to glycine to synthesize GAA. GAA is transferred to the liver, where S-adenosylmethionine (SAM) gives its methyl group to GAA to synthesize creatine. Creatine is then transported by the bloodstream to the target cells with high energy need, such as skeletal muscle, heart, brain, sperm and immune cells. Creatine plays a key role in energy transfer and supply via creatine-phosphocreatine system. Dietary supplemental NutriBuilder® can increase creatine level in the body by 20%. 1234CrCRTATPATPATPATPATPaseANTADPPCrADPADPOPADPANTmtCKGCK-gCKcCKa

Comparison of hygienic indexes among different brandsComparison of body condition after using different brands of GAAItemGAA(%)Cyanamide(mg/kg)Dicyandiamide(mg/kg)Melamine(mg/kg)Dioxin(pg TEQ/kg)≥96%≤100≤2000≤5≤100≥96%≤300≤5000≤20≤750＜96%（variable）2000-200002000-1000020-2001000-5000NutriBuilder®Overseas brandLocal brand① Cyanamide, 300 mg/kg in GAA will cause weight loss, anemia, hypothyroidism, stooping back, tremors, rough fur, pale soft exudative (PSE) meat, nervous disorder and higher death rate.② Dicyandiamide, 2000 mg/kg in GAA degrades into gaseous cyanide access to stomach acid, causes and aggravates respiratory diseases.③ Melamine, above 10 mg/kg in GAA reduces feed consumption, weight loss, bladder stones, crystalluria, renal failure and reduces survival rate.④ Dioxins, above 750 pg TEQ/kg in GAA are extremely harmful to human and animals. Human will suffer reproductive function damage and development problems, immune system damage and cancer after eating even low level of dioxins.2High content of GAA, strict quality control for residues. With a near density (550 kg/m3±10%) as corn and soybean, it can be easily premixed well with feedstuff.3Inferior GAANutriBuilder®

Laying performance in AA﹢broiler breeders from 46 to 50 weeksEnergy sparing effect in broilerFor breederProper dose of GAA in breeder feed can enhance semen quality and sperm motility, improve the fertility and hatchability by 5%-10% and reduce the mortality of embryos, increase growth performance of offspring.ControlReduced 150 kcalReduced 150 kcal+0.06% NutriBuilder®2.72Body weight gain(kg)2.612.712552602652702751.60F:G1.701.611.551601.651701.75Control0.08% GAA0.12% NutriBuilder®Control0.08% GAA0.12% NutriBuilder®72.53Laying rate(%)78.155055606570758077.8263.60Average egg weight(g)63.5263.555052545658606264662.3Feed/egg2.32.282.02.1222.324Hatching performance in AA+broiler breeders from 46 to 50 weeks87.75Qualified egg rate(%)92.6293.158385878991939582.59Fertilization rate(%)93.5094.0376808488929676.32Hatchability(%)88.8089.1265707580859095

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